Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Teknova 1M Tris-HCl, pH 9.2. 1000mL, Sterile. 2 pack.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Biotang Inc Phosphate-buffered saline (PBS, 10X), 2000ml
Phosphate-buffered saline (PBS, 10X), 2000ml.
![Research Products International Corp HEPES, Free Acid [N-(2-Hydroxyethyl) piperazine N'-(2-ethanesulfonic acid)], 250 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-H75030-250.0.JPG-250.jpg)
Research Products International Corp HEPES, Free Acid [N-(2-Hydroxyethyl) piperazine N'-(2-ethanesulfonic acid)], 250 Grams
HEPES, Free Acid (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid), is a zwitterionic buffer.
In the life science field, HEPES, Free Acid, is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures. It is particularly effective in the pH range of 6.8 to 8.2, making it suitable for applications requiring slightly acidic to neutral pH conditions.
HEPES, Free Acid, is commonly used in cell culture media, protein purification, and enzyme assays where a specific pH range needs to be maintained. Its buffering capacity and stability within the desired pH range make it valuable in these applications.
One of the significant advantages of HEPES, Free Acid, is its compatibility with biological systems and enzymes. It exhibits minimal interference with enzymatic activity and other biological processes, ensuring reliable and accurate results.
Electron Microscopy Sciences R-Universal Epitope Recovery Buffer (10x stock) 500 ML
EMS UNIVERSAL Epitope Recovery Buffer is a novel (patent-pending) technology for epitope recovery, primarily based on reversing the fixation effects of formaldehyde, which created links primarily between ε-amino groups of lysine and other amino-groups. In extensive testing the buffer has shown excellent results when used with different antibodies, including those that require low or high pH buffers or protolithic treatment for successful staining.
The EMS Universal Buffer may be used in any heat-induced epitope recovery system (the time and temperature of treatment should be tested), but was specifically adjusted for tissue sections processing in our Antigen Retriever (62700 Series). Using our Universal Buffer in our Retriever guarantees the highest rate of success in recovering epitope for any antibody, especially one that was never previously used on formalin-fixed sections. Shelf Life: 12 months.
Sigma Aldrich Fine Chemicals Biosciences TAPS sodium salt 99 titrat500G
TAPS sodium salt 99 titrat500G
Research Products International Corp TE Buffer, 10X Powder, 158.35 Grams of Powder, Makes 10 Liters of Solution
Dry homogeneous blend of molecular biology grade Tris-base and EDTA, disodium salt. For use in gel electrophoresis.
Supplied as 158.35 grams of powder which is used to produce a 10X concentrate.
Abcam PBS Tablets (1000 ml), 10TABLET
MW 411.04 Da. One of the most commonly used buffers provided in a convenient, tablet form. PBS is isotonic and non-toxic to cells and has the ability to maintain their osmolarity thereby making the buffer suitable for washing procedures in cell cultures and for immunoassays.
The product is subject to the following: Abcam Restricted Use Statement
![Research Products International Corp TRIS Hydrochloride [Tris(hydroxymethyl) aminomethane HCl], 500 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-T60050-500.0.JPG-250.jpg)
Research Products International Corp TRIS Hydrochloride [Tris(hydroxymethyl) aminomethane HCl], 500 Grams
TRIS Hydrochloride, Tris-HCl, is a buffering agent commonly utilized to maintain a stable pH in experimental procedures.
It is primarily used as a buffering agent for applications that require a slightly alkaline to neutral pH range. Also, It is effective in the pH range of 7.2 to 9.0, making it suitable for a variety of biochemical and molecular biology techniques.
TRIS HCl is commonly used in DNA and RNA extraction, protein purification, enzyme assays, and electrophoresis. It helps maintain the desired pH conditions for enzymatic reactions, stability of nucleic acids, and separation of proteins during electrophoresis.
One of the advantages of TRIS HCl is its ability to resist changes in pH caused by the addition of acids or bases, making it a reliable buffering agent. It also exhibits low ionic strength, which minimizes interference with biological systems and enzymatic activities.
![Research Products International Corp TRIS Hydrochloride [Tris(hydroxymethyl) aminomethane HCl], 100 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-T60050-100.02.JPG-250.jpg)
Research Products International Corp TRIS Hydrochloride [Tris(hydroxymethyl) aminomethane HCl], 100 Grams
TRIS Hydrochloride, Tris-HCl, is a buffering agent commonly utilized to maintain a stable pH in experimental procedures.
It is primarily used as a buffering agent for applications that require a slightly alkaline to neutral pH range. Also, It is effective in the pH range of 7.2 to 9.0, making it suitable for a variety of biochemical and molecular biology techniques.
TRIS HCl is commonly used in DNA and RNA extraction, protein purification, enzyme assays, and electrophoresis. It helps maintain the desired pH conditions for enzymatic reactions, stability of nucleic acids, and separation of proteins during electrophoresis.
One of the advantages of TRIS HCl is its ability to resist changes in pH caused by the addition of acids or bases, making it a reliable buffering agent. It also exhibits low ionic strength, which minimizes interference with biological systems and enzymatic activities.
Teknova 50mM Tris, 16% Ethanol, pH 7.5. 1000mL. Endotoxin Tested, Sterile.
50mM Tris,16% (v/v) Ethanol